MIL-PRF-3122J
4.4.3.5 Testing equipment:
HPLC System with variable UV detector and integrating computer. Suitable syringe and
glassware.
A micro-Bondapak C18 column (Waters Associates P/N 2734) or equivalent.
0.45-micron polycarbonate filters (Nuclepore Corporation, Catalog No. 111107) or equivalent.
Calculator capable of doing linear regression.
Reagents: Acetonitrile (Chromatographic grade).
4.4.3.5.1 Typical mobile phase. Mix 550ml of distilled water and 450ml of acetonitrile. Filter
and degas the solution. The concentration may be varied to meet system suitability requirements
within range 40% to 50% acetonitrile.
4.4.3.5.2 Typical chromatographic conditions:
Injection Volume
20 Microliters
Flow
2.5 ml/minute
Detector
235 nm
4.4.3.6 System suitability. Inject the highest working standard until 2 percent agreement is
reached between two successive injections. Chromatograph for approximately 15 minutes.
Determine that, after the solvent front, one impurity peak elutes before the major peak. Elution
for monoiodomethyl-para-tolylsulfone is about 6 minutes and for Diiodomethyl-para-
tolylsulfone is about 8.5 minutes.
4.4.3.7 Procedure. Inject each standard and sample preparation in duplicate allowing each
injection to run for at least 15 minutes. Measure the peak response for the Diiodomethyl-para-
tolylsulfone.
Note: The run time may have to be increased for samples with late eluting peaks. Perform a
linear regression of peak response (area) versus concentration for each of the components.
For each component:
Concentration (ppm) in leather =
Concentration (ppm)
x
75
from linear regression.
Sample wt(g)
4.5 Methods of inspection.
8
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